COVID-19의 항바이러스 인자 셀레늄과 글루타티온 이해: 바이러스 Mpro 프로테아제는 숙주 셀레노 단백질과 글루타티온 합성을 표적으로 합니까?

Impact Factor 3.365 | CiteScore 4.3

Introduction 소개

여러 독립적 연구에서 현재 COVID-19의 결과와 이전에 기록된 중국 도시 (1)의 지역 셀레늄 (Se) 상태, 그리고 유럽 COVID-19 환자 집단에서 혈청 Se와 사망률 간의 유사한 관계 사이에 중요한 연관성을 확립했습니다. (2).

Several independent studies have now established a significant association between the outcome of COVID-19 and previously documented regional selenium (Se) status in Chinese cities (1), and a similar relationship between serum Se and mortality in a European cohort of COVID-19 patients (2).

이러한 관찰은 특히 다양한 RNA 바이러스 (엔테로 바이러스, 한타 바이러스 및 인플루엔자 A) 및 RNA 단계를 가진 바이러스에 대해 수십 년 동안 보고된 Se의 일관된 역할 패턴에 적합하기 때문에 관련된 메커니즘에 대한 질문을 불러 일으 킵니다. (HIV-1 및 B 형 간염 바이러스), 여러 저자가 검토했습니다 (3–5).

These observations invite questions about the mechanisms involved, particularly because they fit into a consistent pattern of a role for Se that has been reported over several decades for a variety of RNA viruses (enteroviruses, hantaviruses, and influenza A) and viruses with an RNA stage (HIV-1 and Hepatitis B virus), as reviewed by various authors (3–5).

셀레노 단백질의 셀레노 시스테인 및 산화 환원 활성 Se- 함유 대사산물로서 Se의 생물학적 역할의 대부분 (전부는 아님)은 단백질 및 펩티드의 시스테인 티올 및 이황화물 및 다양한 산화 형태와의 상호작용을 포함합니다.

Most (but not all) of the biological roles of Se, both as selenocysteine in selenoproteins, and as redox-active Se-containing metabolites, involve interactions with cysteine thiols and disulfides in proteins and peptides, and their various oxidized forms.

Like Se, the essential antioxidant and free radical scavenger glutathione (GSH), a tripeptide thiol, has also proven to be an important factor in various viral infections, particularly in HIV/AIDS, as reviewed in section 2.1.2. of Taylor (6), and most recently, in COVID-19 (7, 8).

Se와 마찬가지로 필수 항산화제 및 자유라디칼 제거제 글루타티온 (GSH), 트리펩티드 티올은 Taylor (6) 섹션 2.1.2에서, 가장 최근에는 COVID-19 (7, 8)에서 검토한 바와 같이 특히 HIV/AIDS에서 다양한 바이러스 감염에 중요한 요인으로 입증되었습니다.

Given their intertwined biochemical roles, there are likely to be common factors and mechanisms underlying the therapeutic importance of Se and GSH in viral infections.

서로 얽힌 생화학적 역할을 감안할 때, 바이러스 감염에서 Se와 GSH의 치료 적 중요성을 뒷받침하는 공통 요인과 메커니즘이 있을 수 있습니다.

SARS-CoV-2 프로테아제와 인간 Selenoprotein 사이의 확인된 분자 상호작용

A Confirmed Molecular Interaction Between SARS-CoV-2 Protease and a Human Selenoprotein

COVID-19 임상 결과와 숙주 Se 및 GSH 상태간 상관관계는 SARS-CoV-2 (SCoV2) 단백질의 가능한 세포 표적에 대한 단백질 체학 기반 연구에서 나오는 관련 관찰에 중요한 맥락을 제공합니다.

Correlations between COVID-19 clinical outcomes and both host Se and GSH status provide important context to a related observation emerging from a proteomics-based study of possible cellular targets of SARS-CoV-2 (SCoV2) proteins.

친화성 정제 질량 분석법을 사용하여 Gordon et al. 26 개의 SCoV2 단백질과 인간 단백질 사이의 신뢰도가 높은 단백질-단백질 상호작용을 확인했습니다 (9).

Using affinity-purification mass spectrometry, Gordon et al. identified high-confidence protein-protein interactions between 26 of the SCoV2 proteins and human proteins (9).

인간 단백질과 상호 작용하는 미끼로 사용 된 단백질 중 하나는 비 구조 단백질 5 (nsp5)로도 알려진 시스테인 프로테아제 인 SCoV2 주요 바이러스 프로테아제 Mpro였습니다.

As bait for interacting human proteins, one of the proteins they used was the SCoV2 main viral protease Mpro, a cysteine protease also known as nonstructural protein 5 (nsp5).

The study also included a catalytically inactive C145A Mpro mutant (lacking the active site cysteine), which was also used as a bait protein, in order to discriminate false positives that might bind non-specifically to the Mpro active site cysteine via disulfide bond formation.

이 연구에는 이황화 결합 형성을 통해 Mpro 활성 부위 시스테인에 비특이적으로 결합할 수 있는 위양성을 구별하기 위해 미끼 단백질로도 사용된 촉매 비활성 C145A Mpro 돌연변이체 (활성 부위 시스테인 결여)도 포함되었습니다.

그들이 확인한 상호작용 중 하나는 세포질 형태의 셀레노 단백질 글루타티온 퍼옥시다아제 GPX1과 관련이 있으며, 이는 비활성 C145A Mpro 돌연변이체에 강하게 결합되었습니다.

One of the interactions they identified involved the cytosolic form of the selenoprotein glutathione peroxidase, GPX1, which bound strongly to the inactive C145A Mpro mutant.

However, this interaction with GPX1 was not observed with the wild type Mpro.

그러나 GPX1과의 이러한 상호작용은 야생형 Mpro에서 관찰되지 않았습니다.

이것은 GPX1이 프로테아제 기질인지 확인하기를 기대할 수 있는 것인데, 그 절단은 반드시 GPX1에 비해 효소에 대한 친 화성이 감소된 2개의 단편을 생성하기 때문입니다.

This is precisely what one might expect to see if GPX1 is a protease substrate, because its cleavage would produce two fragments that would necessarily have reduced affinity to the enzyme relative to GPX1.

확장 데이터 (9)의 그림 6에서 볼 수 있듯이 Gordon 등 비활성 Mpro C145A 돌연변이체에만 결합된 다른 숙주 단백질 (TRMT1)을 확인하고, TRMT1 단백질 서열 (PRLQ/ANFT)에서 추정 Mpro 절단 부위를 식별할 수 있었기 때문에 Mpro 기질일 가능성이 높다고 결론지었는데, 슬래시(/)는 절단 부위를 나타냅니다.

Significantly, as shown in Figure 6 in their Extended Data (9), Gordon et al. identified one other host protein (TRMT1) that bound only to the inactive Mpro C145A mutant, and concluded it was a likely Mpro substrate, because they were able to identify a putative Mpro cleavage site in the TRMT1 protein sequence (PRLQ/ANFT), where the slash (/) represents the cleavage site.

따라서 GPX1에 대해 유사한 결론을 도출하지 못한 유일한 증거는 후보 Mpro 절단 사이트이며, 대부분의 알고리즘은 P1 위치에서 Q를 요구하는 것에 대해 매우 엄격한 경우 찾을 수 없습니다 (그림 1).

Thus, the only piece of evidence lacking to draw a similar conclusion for GPX1 is a candidate Mpro cleavage site, which most algorithms will fail to find, e.g., if they are highly stringent about requiring a Q in the P1 position (Figure 1).

Figure 1. Coronavirus Mpro cleavage site consensus sequence logo plots and comparisons. Logo plots from multiple alignments of the known Mpro cleavage sites are shown for the 2003 SCoV (A) and 2019 SCoV2 (B). The height of a letter at each position reflects its probability in the alignment; each of the logos shown represents the consensus of 11 Mpro cleavage sites from a single virus. Plots were generated using WebLogo (weblogo.berkeley.edu/logo.cgi), from the alignments in Figure S1. (C) Comparison of the GPX1 active site sequence containing selenocysteine (U) to the known SCoV2 Mpro cleavage site at the nsp13/14 junction; this site is identical in the 2003 and 2019 coronaviruses. (D) Comparison of a predicted Mpro cleavage site in human selenoprotein F to a known SCoV2 Mpro cleavage site at the nsp12/13 junction.

As shown in Figure 1A for the 2003 SARS coronavirus (SARS-CoV, SCoV) and in Figure 1B for the 2019 SCoV2, the consensus logo patterns of 11 Mpro cleavage sites in the viral 1ab polyprotein are essentially identical for the 2 viruses. Specifically, 8 of the 11 sites are fully identical over the 10 residues spanning the P5 to P5′ positions, and only two of the 11 cleavage sites have more than a single amino acid difference within the 10 residue span (Figure S1). This means that (as discussed below) functional studies of the 2003 SCoV protease are highly relevant to that of the 2019 virus. From Figures 1A,B, the most important residue positions for Mpro recognition are, in descending order, P1, P2, P1′, P4, and P3, with the first 3 being the major determinants, with the minimal P2-P1′ consensus being LQ/(S,A,N,G). The downstream sequence past P1′ is highly variable.

The GPx1 active site has the sequence LUG, where U is the catalytic selenocysteine; this matches the observed Mpro consensus core target sequence combination LQ/G in 2 of 3 positions. Furthermore, as shown in Figure 1C, the important upstream side of the known Mpro cleavage site at the nsp13/14 junction, NVATLQ/A, is remarkably similar to that of the active site sequence of GPx1, NVASLU/G, where the selenocysteine (U) lines up with a glutamine (Q) in the viral sequence. These two amino acids (U and Q) are not highly similar, but are both midrange in size, and polar in nature, because the selenol residue is predominantly ionized at physiological pH. The other two “mismatches” in the important positions P3 and P1′, i.e., S vs. T and G vs. A, both differ only slightly, by the size of a methyl group, and in any case, having a glycine (G) in the P1′ position is a permitted residue (Figure 1A), as observed in the SCoV Mpro cleavage site at the nsp5/6 junction (indicated by * in Figure 2). Significantly, the Procleave protease cleavage prediction server (procleave.erc.monash.edu.au) (13) identified the GPX1 active site octameric sequence ASLU/GTTV as a highly ranked possible cleavage site for a cathepsin S-like cysteine protease (Figure 2G).

FIGURE 2

Figure 2. Comparison of known and predicted Mpro cleavage sites. The most relevant positions P4-P1′ of the 11 known SARS coronavirus Mpro cleavage sites in non-structural proteins are shown (for nsp4 through nsp16), aligned with predicted sites in several human selenoproteins (GPX1, SelenoF, SelenoP, and TXNRD1), as well as glutaredoxin-1 (GLRX-1) and the rate-limiting enzyme for glutathione synthesis, glutamate-cysteine ligase catalytic subunit (GCLC, with 2 predicted cleavage sites A, B). The known nsp cleavage sites shown are all from SARS-CoV-2, with the addition of the nsp5/6 site from SARS-CoV (marked*). The sites identified in human proteins are displayed next to the known Mpro cleavage site to which they are most similar, highlighted in matching color. The experimentally determined catalytic efficiencies of SARS-CoV Mpro at each of the known nsp cleavage sites are shown as relative kcat/Km (10). Three different methods for prediction of cysteine protease cleavage sites were used: PROSPER (11), with numbers in parenthesis showing the score for the predicted site, any score >0.8 being significant; NetCorona (12), where scores >0.5 are considered positive, and Procleave (13), where every possible P4-P4′ octamer in a protein sequence is evaluated, scored and ranked. Some aligned non-identical residues highlighted in italics are nonetheless chemically and structurally similar to residues found in the same position in the known cleavage sites, e.g., serine (S) vs. threonine (T), Leucine (L) or isoleucine (I) vs. valine (V), and glutamine (Q) vs. asparagine (N); all of these pairs differ only by a single carbon atom (CH3 or CH2 unit). The predicted Mpro cleavage sites in human proteins labeled (A–G) are discussed in the text. The computational results cited are collated in Figure S2.

For several reaction intermediates in the GPX1 mechanism, Se is attached to either oxygen, or to nitrogen, as a selenenylamide. The latter is quite stable, and may accumulate during GSH depletion, because GSH is needed to regenerate the selenolate (14). Selenenylamide is more similar than selenocysteine to glutamine, so that form might be preferentially targeted by Mpro.

Because the mutation rate in cellular genes is thousands of times slower than that for an RNA virus, if there is a physical interaction between these two proteins as Gordon et al. observed using the inactive mutant Mpro, it is almost certainly because the virus has evolved to target the host for some reason, rather than the converse. The most parsimonious hypothesis is simply that this is a case of a viral protease targeting a cellular gene for cleavage, for which there are abundant precedents, including the proposed Mpro targeting of TRMT1 and HDAC2 (9), and HIV-1 protease, which can be toxic to cells, via its action at various cellular targets (15).

Functional Effects of MPRO in Transfected Cells are Consistent With GPX1 Knockdown

As detailed above, the original 2003 SCoV Mpro has essentially identical substrate sequence specificity as that of SCoV2. More specifically, the 10 residue sequence of the cleavage site at the nsp13/14 junction shown in Figure 2A, with the greatest overall similarity to the GPX1 active site sequence, is 100% identical in the 2003 and 2019 SARS coronaviruses (Figure S1). Thus, the results of a 2006 study on the effects of transfecting cells with a SCoV Mpro expression vector, in order to study the effects of Mpro alone in the absence of intact virus (16), are highly relevant for COVID-19. In transfected human cells, the results were exactly what one would expect from knockdown of GPX1: increased oxidative stress via production of reactive oxygen species and activation of transcription factor NF-κB (16). Both of these responses can be produced by exposing cells to hydrogen peroxide, and inhibited by increasing GPX1 activity, either by overexpression or via the addition of sodium selenite to cell culture media (17, 18). Cells expressing the SCoV Mpro also became apoptotic, which is a known consequence of NF-κB activation, especially in combination with a high burden of ROS (19), which is even more likely to occur if other antioxidant proteins are also degraded, as discussed below.

Potential MPRO Sites in Other Selenoproteins and Glutathione-Related Proteins

The question then arises, could targeting of host selenoproteins for proteolysis be part of a more extensive viral strategy contributing to the correlations between Se and GSH status and COVID-19 outcome? To investigate this possibility, we undertook a systematic search for potential Mpro cleavage sites in the human selenoproteome and in several GSH-related proteins including glutaredoxin, a small thioredoxin-like CXXC protein that is recycled by GSH, rather than directly by a reductase enzyme. For this analysis, we used three online resources: (1) NetCorona (cbs.dtu.dk/services/NetCorona) (12), the only resource specific for predictions of Mpro cleavage sites in coronaviruses (although not limited to just the SARS type coronaviruses), (2) PROSPER, the Protease Specificity Prediction Server (prosper.erc.monash.edu.au) (11), which was rated in a recent study as one of the most accurate resources for prediction of HIV-1 protease sites (20), and (3) Procleave (cited above), which was only used if either of the first 2 methods failed to identify a site. Both Procleave and PROSPER do not specifically predict coronavirus cysteine protease sites, but make predictions for more generic cysteine proteases (e.g., cathepsins B, K, L, or S). Thus, for both PROSPER and Procleave, the results had to be screened manually for matches to the Mpro cleavage site consensus as shown in Figures 1A,B. This search is also predicated on the assumption that the approach of Gordon et al. (9) might fail to identify some protease targets, because their high affinity interactions are mostly limited to an 8–10 residue sequence, and in some cases may be unable to withstand the conditions needed to be isolable and observable by mass spectrometry.

Figure 2 presents the most notable and highly ranked potential Mpro sites identified computationally (the order A-G is not significant, as placement in the listing was determined by similarity to the known nsp sites). The most remarkable instance is in selenoprotein F (SELENOF), an ER protein involved in glycoprotein folding quality control. Over the 8 residues spanning P4 to P4′, it has 7 of 8 identical to the Mpro site at the nsp12/nsp13 junction, with the only mismatch at the highly variable P3′ position (Figure 1D), giving a near-perfect match to the Mpro consensus. This was the highest scored NetCorona hit, also highly scored by PROSPER, as shown in Figure 2F, and is significant because coronavirus assembly begins in the ER with spike protein accumulation on the ER membrane surface.

Equally important is the hit on the selenoprotein thioredoxin reductase 1 (TXNRD1), which also was highly scored by both NetCorona and PROSPER (Figure 2E). Note that in a larger set of 77 human coronaviruses, a serine (S) in P4 is very common (12), and in addition the sequence SI in the TXNRD1 site is isosteric to TV, the aligned residues in the nsp12/13 site, because transfer of a methyl group from threonine to valine would result in serine and isoleucine. Thus, SI should occupy about the same volume as TV in the Mpro active site. Functionally, this predicted cleavage site is only 5 residues from the C terminal of TXNRD1, and would result in removal of the C-terminal redox center of TXNRD1 (AGCUG), making the enzyme incapable of regenerating reduced thioredoxin.

A predicted site in selenoprotein P (SELENOP, Figure 2C) is at position 56, 3 residues prior to the first selenocysteine, such that cleavage here would likely interfere with the N-terminal redox activity, possibly without affecting the Se-transport function of the rest of the protein.

Two possible sites were identified in the catalytic subunit of glutamate-cysteine ligase (GCLC), the rate limiting enzyme for GSH synthesis. The first of these, GCLC-a (Figure 2B), would cleave after position 217, and is an exact match to P4-P1 of nsp5/6, but with an A in P1′, a highly favorable residue for that position, although SCoV has a G there, and SCoV2 has an S. Site GCLC-b (Figure 2A), at position 443, is an exact match to P4-P1 of nsp4/5, but with a G in P1′. The two coronavirus sites with exact P4-P1 matches to GCLC are those at either end of nsp5, i.e., Mpro itself, which have the highest catalytic efficiency for cleavage of any of the viral Mpro targets (100 and 41%). This suggests that Mpro might be particularly efficient at disrupting GSH synthesis, as compared to action at some of the other target sites predicted here, thereby contributing to the clinical findings in COVID-19 (7).

Finally (Figure 2D), we include a non-canonical hit that would cleave at position 90 of glutaredoxin-1 (GLRX-1). This site was very highly scored by PROSPER, as well as Procleave, despite the glutamine (Q) at P1′, which has an additional carbon atom relative to the asparagine (N) found at P1′ in the nsp8/9 site, to which it is otherwise most similar.

Why Would SARS-CoV-2 Target Components of the Thioredoxin and Glutaredoxin Systems?

Along with GSH reductase, GSH and glutaredoxin comprise the glutaredoxin system, one of two cellular redox systems involved in maintaining reduced thiol states, protein folding and repair, and providing electrons to ribonucleotide reductase (RNR). The other redox system with similar roles is the thioredoxin system, comprised of thioredoxin and thioredoxin reductase (21).

Because DNA is an “add-on” to RNA biosynthesis, deoxyribonucleotides can only be synthesized via the reduction of ribonucleotides by RNR, a process which is unsustainable without the participation of one or both of the thioredoxin and glutaredoxin systems. As a consequence of this basic fact of biochemistry, one should expect that RNA viruses might exploit various mechanisms to interfere with components of the thioredoxin and glutaredoxin systems, in order to minimize the diversion of ribonucleotides into DNA synthesis. This is simply the inverse of a strategy used by some large DNA viruses to maximize DNA production, by encoding their own thioredoxin-like proteins, glutaredoxins and even entire RNR genes (22). Consistent with this hypothesis, the results presented here suggest coronaviral targeting of TXNRD1, glutaredoxin-1, and GCLC, a key enzyme for GSH synthesis, for proteolytic cleavage. This would lead to knockdown of both of the essential redox systems required to sustain DNA synthesis, and thereby conserve ribonucleotides to enhance RNA synthesis for virus production.

The Potential MPRO Targets TXNRD1 and GCLC are Strongly Upregulated by Vitamin D3

In light of accruing evidence that vitamin D3 status is inversely correlated with severity of COVID-19 (23–26), it is significant that, via its hormone-like actions on gene expression, D3 has been shown to be a potent activator of both TXNRD1 and GCLC. In a microarray study, 6 h after exposure to 1,25-dihydroxyvitamin D3, TXNRD1 mRNA was upregulated 7.1X, and GCLC was upregulated 2.5X (27). These effects of vitamin D3, as well as a resulting increase in GSH levels, have been functionally documented in cell culture studies (28, 29) and in human subjects (30). Vitamin D3-mediated activation of both TXNRD1 and GSH biosynthesis could substantially counteract the proteolytic knockdown of TXNRD1 and GCLC predicted by our hypothesis, which, if validated, would thus represent an important mechanism contributing to a role for vitamin D3 in moderating the severity of COVID-19. However, the therapeutic potential of D3 in COVID-19 is the subject of ongoing debate (31). Perhaps inconsistencies in the findings of various studies of this question may be explained in part by a 20-year old observation, that the effective upregulation of TXNRD1 by vitamin D3 requires the presence of an adequate level of Se (28). So the full potential of vitamin D3 vs. COVID-19 may only be seen in combination with optimal Se intake, and possibly, vice-versa.

Discussion and Conclusions

Altogether, given the protein interaction and functional data (9, 16), it is a very strong hypothesis that GPX1 is an Mpro substrate. Of the other sites we propose, some are more likely than others, but those in TXNRD1 and SELENOF are particularly convincing, and all of the predicted sites map to surface accessible regions of the targeted proteins (Figure S3). If validated, these results offer new insights into COVID-19 pathogenesis. If SCoV2 is targeting GSH biosynthesis as well as TXNRD1 and GPx1 for proteolytic knockdown, in infected cells, the resulting decreases in these critical antioxidant molecules would contribute to increased oxidative stress, NF-κB activation and pro-apoptotic signaling (17, 18). Because NF-κB is an activator of many pro-inflammatory cytokines, including IL-6 (32), this could contribute to increased inflammation and the cytokine storms observed in COVID-19, and be a significant basis of pathogenic effects associated with SCoV2 infection of various tissues, including the lung, gastrointestinal tract and cardiovascular system. Importantly, these consequences of virus-mediated proteolysis would be taking place in everyone who is actively infected, regardless of their Se status, and would thus be consistent with results suggesting that Se intakes up to several times the minimal dietary requirement are associated with an increase in cure rate from COVID-19 (1). But people with suboptimal nutritional status could be particularly at risk, because their GSH and selenoprotein levels might be low to begin with, making them more vulnerable to the detrimental effects of virus-induced proteolysis. Still, an infection resulting from low dose exposure to virus and limited by a strong immune response in a person with excellent dietary status still might have minimal impact on cellular and patient health, because proteolytic knockdown of host proteins is likely to be incomplete, due to low catalytic efficiencies at some target sites (Figure 2) and the stochastic nature of such molecular interactions.

These results are not unprecedented—our lab has shown that some RNA viruses target host mRNAs encoding isoforms of thioredoxin reductase via RNA:RNA antisense interactions, which, like proteolysis, would likely also result in host selenoprotein knockdown, but by a different mechanism (22, 33). If our hypothesis is confirmed (i.e., some of these host cleavage sites prove to be functional), that leaves us with an interesting question—what evolutionary advantages are driving some viruses to go so far as to degrade or block the synthesis of GSH and specific host selenoproteins?

Understanding why RNA viruses may have developed such strategies presents an interesting challenge for future research.

Data Availability Statement

All datasets generated for this study are included in the article/Supplementary Material.

Author Contributions

This study was conceived and designed by ET, who wrote the initial draft. ET and WR worked on the data analysis and revisions of the manuscript. All authors contributed to the article and approved the submitted version.

Funding

This work has been supported by a recurring unrestricted gift from the Dr. Arthur and Bonnie Ennis Foundation, Decatur, IL, USA.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fnut.2020.00143/full#supplementary-material

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노화방지 NMN 스킨케어 헤어

COVID-19의 항바이러스 인자 셀레늄과 글루타티온 이해: 바이러스 Mpro 프로테아제는 숙주 셀레노 단백질과 글루타티온 합성을 표적으로 합니까?

Introduction 소개

여러 독립적 연구에서 현재 COVID-19의 결과와 이전에 기록된 중국 도시 (1)의 지역 셀레늄 (Se) 상태, 그리고 유럽 COVID-19 환자 집단에서 혈청 Se와 사망률 간의 유사한 관계 사이에 중요한 연관성을 확립했습니다. (2).

SARS-CoV-2 프로테아제와 인간 Selenoprotein 사이의 확인된 분자 상호작용

A Confirmed Molecular Interaction Between SARS-CoV-2 Protease and a Human Selenoprotein

'리포조말 비타민 글루타치온 콜라겐' 카테고리의 다른 글

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티스토리툴바

COVID-19의 항바이러스 인자 셀레늄과 글루타티온 이해: 바이러스 Mpro 프로테아제는 숙주 셀레노 단백질과 글루타티온 합성을 표적으로 합니까?

Introduction 소개

​여러 독립적 연구에서 현재 COVID-19의 결과와 이전에 기록된 중국 도시 (1)의 지역 셀레늄 (Se) 상태, 그리고 유럽 COVID-19 환자 집단에서 혈청 Se와 사망률 간의 유사한 관계 사이에 중요한 연관성을 확립했습니다. (2).

SARS-CoV-2 프로테아제와 인간 Selenoprotein 사이의 확인된 분자 상호작용

A Confirmed Molecular Interaction Between SARS-CoV-2 Protease and a Human Selenoprotein

'리포조말 비타민 글루타치온 콜라겐' 카테고리의 다른 글

관련글

댓글

티스토리툴바

여러 독립적 연구에서 현재 COVID-19의 결과와 이전에 기록된 중국 도시 (1)의 지역 셀레늄 (Se) 상태, 그리고 유럽 COVID-19 환자 집단에서 혈청 Se와 사망률 간의 유사한 관계 사이에 중요한 연관성을 확립했습니다. (2).